Figure 4.

Galangin decreased glioma growth in an orthotopic xenograft mouse model. U87-luciferase cells (5×10⁵) were intracranially injected into the mid-right striatum of 8-week-old male BALB/c nude mice. Seven days after injection, the tumor formation was examined by bioluminescence imaging, and the mice were separated into two groups: those that were intraperitoneally injected with vehicle (control) and those that were intraperitoneally injected with galangin (200 mg/kg/day). Tumor sizes were measured once every 9 days. Bioluminescence imaging was used to measure tumor volume. (A) A representative mouse demonstrating the tumor volume from each group is shown at each time point. (B) The tumor volume of each group was examined at each time point. (C) The survival rate of each group was recorded. (D) The body weight of the mice was measured at the indicated time points. At the end of the experiment or after the mice died, the brains were excised (E) and CD44 (bar, 20 μm) and (F) VWF (bar, 50μm) were stained. The images were analyzed by Image Pro-Plus. (G) At the end of the experiment or after the mice died, tumor tissues were excised from the mice, and protein lysates were performed to estimate the protein expression. *P<0.05, compared with control.