Figure 3.

Effects of RAGE-aptamer on CML-RAGE interaction in vitro (a and b) and ROS generation (c–e) in CML-exposed G361 melanoma cells. a and b: representative curves of QCM sensors. Binding affinity of CML (a) or RAGE-aptamer (b) to vRAGE immobilized on a QCM surface in the presence or absence of RAGE-aptamer. N = 3 for each group. (c–e) G361 cells were treated with or without 10 μM carboxy-H₂DFFDA for 30∼60 minutes. Then, G361 cells were incubated with the indicated concentrations of CML in the presence or absence of the indicated concentrations of RAGE-aptamer for 25 minutes. Intracellular ROS production in G361 cells was measured with a fluorescent probe, carboxy-H₂DFFDA. (c) N = 6 for each group. (d, e) N = 4 for each group. ^∗p < 0.05 and ^∗∗∗∗p < 0.01 compared with the control values without CML. ##, p < 0.01 compared with the values with CML alone.