Table 2.
Effects of resveratrol, AMPK activator (AICAR), and inhibitor (Compound C) on sperm motility parameters in the H2O2-induced oxidative stress model in vitro.
| Sperm parameters | FS | H2O2 | H2O2+R | H2O2+AICAR | H2O2+R+CC |
|---|---|---|---|---|---|
| TM (%) | 80.9 ± 6.5a | 58.5 ± 9.6c | 64.3 ± 2.2b | 68.4 ± 8.8b | 65.5 ± 9.0b |
| PM (%) | 62.8 ± 5.2a | 38.9 ± 4.1d | 52.7 ± 2.0b | 53.3 ± 2.6b | 46.5 ± 5.9c |
| LIN (%) | 46.8 ± 2.5a | 37.7 ± 3.5c | 43.4 ± 1.4a | 43.6 ± 1.1a | 44.9 ± 3.4a |
| VSL (μm/s) | 80.3 ± 4.4a | 56.4 ± 8.8d | 73.2 ± 4.3b | 72.8 ± 6.1b | 64.0.5 ± 6.4c |
| VCL (μm/s) | 171.9 ± 3.4a | 136.3 ± 14.2b | 169.3 ± 11.4a | 165.5 ± 11.0a | 167.9 ± 8.4a |
| VAP (μm/s) | 101.9 ± 1.9a | 82.0 ± 19.7c | 95.5 ± 5.6ab | 94.1 ± 5.3ab | 91.6 ± 4.2b |
| BCF (Hz) | 30.9 ± 1.0a | 30.9 ± 3.0a | 28.0 ± 2.7a | 30.9 ± 0.9a | 29.1 ± 2.0a |
Values are expressed as mean ± SEM. Different letters within the column indicate significant difference (p < 0.05). TM: total motility; PM: progressive motility; VCL: curvilinear velocity; VSL: straight-line velocity; VAP: average path velocity; BCF: beat-cross frequency; LIN: linearity (VSL/VCL); FS: fresh sperm; R: resveratrol; CC: Compound C.