. Author manuscript; available in PMC: 2019 Sep 16.

Published in final edited form as: Dev Dyn. 2019 May 2;248(7):583–602. doi: 10.1002/dvdy.43

Table 4.

List of primers used for in situ hybridization.

Gene ID	Symbol	Forward primer	Reverse primer

NCC genes
395245	MSX2¹	5’-CGAGGAGCACCACAAAGTCAAG-3’	5’-GACAGGAGTAGCATAGAGTCCAACG-3’
432368	SNAI2	5’-CTGTGTGGACTACAACCGGG-3’	5’-CTTCACATCCGAGTGGGTCT-3’
395734	RHOB	5’-TCGTCTTCAGCAAGGACGAG-3’	5’-GCAATTGATGCAGCCGTTCT-3’
RA genes
416880	ALDH2	5’-ATCCCTTGGAACTTCCCCCT-3’	5’-ACTGATGCCCAACAGCAACT-3’
419480	DHRS3	5’-CCTTCCCTTCCTTCGCTTTAT-3’	5’-GTGCTTCCAAACTCCACATTTC-3’
386585	NR2F2	5’-CAAAGTTGGCATGAGACGGG-3’	5’-AGCTTCCCGAATCGTGTTGG-3’
TGF-β
421461	LTBP1²	5’-TGCATCAAACCTAACTGTGCA-3’	5’-TCGGAAGTTAGTGGCTGTCA-3’
428413	BAMBI³	5’-GATCGCCATTCCAGCTACAT-3’	5’-TTTGCTGTCGTTGATCTTGC-3’
395897	TGFBI	5’-CACACAGCTCTACTCCGACC-3’	5’-GGCCAACTCAAACAGGGTCT-3’
Wnt genes
100858542	AES	5’-TGTTTCCACAAAGCCGACAC-3’	5’-TTCTCCCCATCGTCGTCTTG-3’
424707	WLS	5’-AGTGATCGCCTTTCTGGTGG-3’	5’-GCTATGGGTCCAACCTGCTT-3’
395862	PITX2	5’-AGCGGACGCACTTCACCAGC-3’	5’-CGCAGCTCAGTCCGTGGCAA-3’