Figure 4.

mAb27 staining of human cell lines shows a similar pattern as commercial (polyclonal) anti-hP2X4 Abs. The human prostate cancer cell line DU145 (A) or immortal human bronchial epithelial cells (16HBE14o–) (B), were stained with mAb 27 or with two polyclonal anti-P2X4 Abs from Santa Cruz and Abnova. P2X4 localization was visualized by confocal microscopy. Staining with secondary antibodies alone was negative (Figure S2). The images are representative of three independent experiments.