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. 2019 Sep 6;10:2074. doi: 10.3389/fimmu.2019.02074

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Copyright © 2019 Paalme, Rump, Mädo, Teras, Truumees, Aitai, Ratas, Bourge, Chiang, Ghalali, Tordjmann, Teras, Boudinot, Kanellopoulos and Rüütel Boudinot.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analyses of hP2X4 expression by CD45⁺ PBL. The gating strategy is presented in (A–C). CD45⁺ leukocytes were stained with fluorochrome-coupled anti-P2X4 mAb27 and with anti-CD3, anti-CD20 and anti-CD14 mAbs. Representative dot plots show P2X4 vs. CD3 (D), P2X4 vs. CD20 (E) and P2X4 vs. CD14 (F) expressions within the CD45⁺ leukocyte population. A representative dot plot shows the expression of P2X4 vs. side scatter (SSC) and identifies a highly granular P2X4 ^high subpopulation (G). This subpopulation represented about 2% of the CD45⁺ leukocyte population. Quadrants were placed based on the fluorescent levels observed in isotype controls. Additional controls are shown on Figure S3.