Figure 7.

Eosinophils from human PBL express highest levels of P2X4. CD45⁺ peripheral blood leukocytes defined by the gating strategy presented in (A–C) were stained with fluorochrome-coupled anti-P2X4 mAb27 and with anti-Siglec8 mAb, and analyzed by flow cytometry. A representative dot plot shows P2X4 vs. SIGLEC8 expression (D) in CD45⁺ leukocytes. Three gates outlined in red (gate 1), blue (gate 2), and green (gate 3) define P2X4^highSIGLEC8^high, P2X4^medSIGLEC8^low, and P2X4^lowSIGLEC8^low subsets, respectively. (E,F) Represent forward (FSC) (respectively, CD45 expression) vs. side scatter (SSC) for cells from each gate, showing that P2X4^highSIGLEC8^high are large, highly granular cells.