Figure 5. ICAM-2 WT and mAB variants inhibit NB cell adhesion to ECM proteins.
(A) NB cells adherent to each ECM protein were imaged after staining. The optical density for Control cells (used in calculations and presented as 100%) were for fibronectin=0.5630 ± 0.0032, for collagen I=0.4855 ± 0.019, and for vitronectin=0.4443 ± 0.0160 (mean ± SEM), as shown in right panel. (B) NB cell adhesion was quantitated by determination of the optical density of crystal violet-stained, adherent cells by standard methods44. Values for ICAM-2 transfectants were significantly different from Control transfectants (**P<0.001 or ***P<0.0001, as indicated).