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. Author manuscript; available in PMC: 2019 Sep 16.
Published in final edited form as: Oncogene. 2014 Apr 7;34(12):1553–1562. doi: 10.1038/onc.2014.87

Figure 6. ICAM-2 WT and mAB variants inhibit NB cell motility in Boyden chamber migration assays.

Figure 6.

(A) Transfected NB cells that migrated to the distal side of Boyden chamber membranes were imaged after an 18-hour incubation. Representative images were acquired with a Zeiss Axio Observer Z.1 microscope and Zen 2011 Blue software. (B) Migrated cell numbers were determined manually, and analyzed by t-test. ICAM-2 WT, mAB4 and mAB8 inhibited cell motility toward fibronectin compared to cells that expressed no detectable ICAM-2 (Control) (P<0.05*), in an α-actinin-independent manner. (C) ICAM-2 WT, mAB4 and mAB8 inhibited cell migration using collagen I as chemoattractant in an α-actinin-dependent manner (*P=0.0365; **P=0.0018; ***P<0.0001; *P=0.0155; ***P<0.0001).