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. Author manuscript; available in PMC: 2019 Sep 16.
Published in final edited form as: Cell Rep. 2019 Sep 3;28(10):2608–2619.e6. doi: 10.1016/j.celrep.2019.07.098

Figure 3. Transcriptomic and Enzymatic Measurements Identify Glutathione Stress in MPC LivKO Tumors.

Figure 3.

(A) Unsupervised clustering of significantly changed gene expression in tumors compared to paired normal adjacent tissue; n = 4 biological replicates.

(B) Venn diagram of gene expression clusters with glutathione-related and HCC marker genes.

(C) Significantly changed pathways based on RNA-sequencing-derived gene clusters identified by Ingenuity Pathway Analysis (IPA). The IPA pathways are identified in different groups because different genes in the same IPA pathway are changed between groups.

(D) Total glutathione (GSH + GSSG) levels measured in paired normal-adjacent and tumor tissue taken from WT and MPC LivKO mice at euthanasia; n = 15–17 biological replicates.

(E) The data in (D) presented as mean change in total glutathione levels; n = 15–17 biological replicates.

(F) Reduced glutathione (GSH) to oxidized glutathione (GSSG) ratios in WT and MPC LivKO normal-adjacent and tumor tissue. Ratios are normalized to WT NormAdj levels. n = 15–17 biological replicates. For statistical analysis, data were analyzed by two-way ANOVA.

Data are presented as mean ± SEM, compared by t test performed unless otherwise noted (*p < 0.05, **p < 0.01, ***p < 0.001).