| Mpc1 |
Cell Signaling |
Cat#14462; RRID: AB_2773729 |
| Mpc2 |
Cell Signaling |
Cat#46141; RRID: AB_2799295 |
| VDAC |
Cell Signaling |
Cat#4664; RRID: AB_10557420 |
| Cleaved Caspase-3 |
Cell Signaling |
Cat#9661; RRID: AB_2341188 |
| Ki67 |
Abcam |
ab#16667; RRID: AB_302459 |
| Goat anti-Rabbit DyLight 800 |
ThermoFisher |
SA5-35571; RRID: AB_2556775 |
| Goat anti-Mouse DyLight 800 |
ThermoFisher |
SA5-10176; RRID: AB_255675 |
| DAKO EnVision + Dual Link System-HRP (DAB+) |
Dako |
K4065 |
| Bacterial and Virus Strains |
|
|
| AAV8.TBG.PI.Cre.rBG |
UPenn Viral Vector Core |
AV-8-PV1090 |
| AAV8.TBG.PI.Null.bGH |
UPenn Viral Vector Core |
AV-8-PV0148 |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| Penicillin-Streptomycin |
ThermoFisher |
15140-122 |
| Insulin |
Sigma |
I9278 |
| Dexamethasone |
Sigma |
D4902 |
| Capillary blood tubes |
Sarstedt |
16.444.100 |
| Fetal Bovine Serum |
Atlanta Biologicals |
S11150 |
| GlutaMAX |
GIBCO |
3505006 |
| Protease Arrest |
G Biosciences |
786-437 |
| Igepal CA-630 |
Sigma |
I8896 |
| N-nitrosodiethylamine (DEN) |
Sigma |
N0258 |
| Trizol |
Life Technologies |
15596018 |
| High-Capacity cDNA Reverse Transcription Kit |
Applied Biosystems |
4368814 |
| SYBR Green ER SuperMix |
Life Technologies |
11760-100 |
| DNeasy Kit |
QIAGEN |
#69504 |
| Benzonase |
Sigma |
E1014 |
| Alkaline Phosphatase |
ThermoFisher |
EF0654 |
| miRNeasy |
QIAGEN |
#217004 |
| Sulfosalicylic acid |
Sigma |
90275 |
| Pierce BCA Assay Kit |
ThermoFisher |
23225 |
| 2-vinylpyridine |
Sigma |
132292 |
| Resazurin |
Sigma |
R7017 |
| UK5099 |
Tocris |
4186 |
| L-Buthionine-sulfoximine |
Sigma |
B2515 |
| N-acetyl cysteine |
Sigma |
A9165 |
| Monochlorobimane |
Sigma |
69899 |
| U-13C Glutamine |
Cambridge Isotopes |
CLM-1822 |
| Methyoxyamine hydrochloride (MOC) |
Sigma |
226904 |
| N,O-Bis(trimethylsilyl)trifluoroacetamide (TMS) |
Sigma |
155195 |
| Carbon Tetrachloride (CCl4) |
Sigma |
289116 |
| Dihydroethidium |
ThermoFisher |
D1168 |
| Williams E Media |
ThermoFisher |
12551-032 |
| Critical Commercial Assays |
|
|
| Glutathione Assay Kit |
Sigma |
CS0260 |
| DNA/RNA Oxidative Damage ELISA Kit |
Caymen Chemical |
589320 |
| ALT Activity Assay |
ThermoScientific |
TR18503 |
| AST Activity Assay |
ThermoScientific |
TR70121 |
| Crystal Violet Cell Cytotoxicity Assay Kit |
BioVision |
K329-1000 |
| Deposited Data |
|
|
| RNA-sequencing of paired normal-adjacent and liver tumor tissue from WT and Liver MPC-knockout mice |
This paper |
GEO: GSE132728
|
| Tissue Cancer Genome Atlas |
http://www.cbioportal.org/ |
N/A |
| Experimental Models: Cell Lines |
|
|
| Hepa1-6 |
Sigma |
92110305 |
| Experimental Models: Organisms/Strains |
|
|
| Mouse: WT: Mpc1fl/fl: C57Bl6/J-Mpc1fl/fl
|
Gray et al., 2015 |
N/A |
| Mouse: MPC LivKO: Mpc1fl/fl: C57Bl6/J-Mpc1fl/fl+AlbCre
|
Gray et al., 2015 |
N/A |
| Oligonucleotides |
|
|
| Mpc1 Forward Primer: AACTACGAGATGAGTAAGCGGC |
IDT |
N/A |
| Mpc1 Reverse Primer: GTGTTTTCCCTTCAGCACGAC |
IDT |
N/A |
| Mpc2 Forward Primer: CCGCTTTACAACCACCCGGCA |
IDT |
N/A |
| Mpc2 Reverse Primer: CAGCACACACCAATCCCCATTTCA |
IDT |
N/A |
| 36b4 Forward Primer: CGTCCTCGTTGGAGTGACA |
IDT |
N/A |
| 36b4 Reverse Primer: CGGTGCGTCAGGGATTG |
IDT |
N/A |
| Software and Algorithms |
|
|
| Excel |
Microsoft |
N/A |
| SigmaPlot |
Systat Software |
N/A |
| GraphPad Prism |
Graphpad Software Inc. |
N/A |
| HiSAT2 |
https://ccb.jhu.edu/software/hisat2/index.shtml |
N/A |
| Partek Genomics Suite |
Partek Inc. |
N/A |
| Ingenuity Pathway Analysis |
QIAGEN |
N/A |
| Cluster 3 |
http://bonsai.hgc.jp/~mdehoon/software/cluster/software.htm |
N/A |
| Java TreeView |
http://jtreeview.sourceforge.net |
N/A |
| ClustVis |
https://biit.cs.ut.ee/clustvis/ |
N/A |
| Other |
|
|
| Luna aminopropyl LC column |
Luna aminopropyl LC column |
Luna aminopropyl LC column |
