Skip to main content
. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Biomaterials. 2019 Aug 23;222:119451. doi: 10.1016/j.biomaterials.2019.119451

Figure 5. Extent of HA modification influences early chondrogenesis in 3D hydrogels.

Figure 5.

(a) Human MSCs were encapsulated in HA hydrogels from NorHA1 macromers (red) using a thiolene reaction, as well as PEG hydrogel controls (grey). After 3 days of culture, samples were analyzed for (b) live-dead staining (scale bar 250 μm) and gross appearance of individual specimens (inset scale bar 5 mm), and the expression of (c) collagen II (COL2), (d) aggrecan (ACAN), and (e) SOX9 genes (reported normalized to GAPDH and 2D control cells prior to encapsulation). n=4 hydrogels per group, * P < 0.05, ** P < 0.01; # P < 0.05 and ## P < 0.01 relative to the PEG hydrogel control.