(A) Illustration depicting experimental setup and timeline to assay adult Drosophila grooming behavior. For all genotypes, flies were tested at 10 and 25 days to assess grooming duration and grooming bout length. All analysis was performed blinded to genotype and in random order.
(B) Quantification of percent of time spent grooming during a 3-min trial. There were no significant differences among wild-type, Wnd, or dFmr1,Wnd double mutants at any age.
(C) Quantification of average bout length within each 3-min trial at each age. There were no significant differences among wild-type, Wnd, or dFmr1,Wnd double mutants at any age.
*p = 0.05; **p = 0.01; ***p = 0.001; ****p = 0.0001; NS, not significant; p > 0.05. p values shown on figure represent statistical comparison to wild-type control within the same testing age.
Detailed statistical tests and additional comparisons available in Table S1.