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. Author manuscript; available in PMC: 2019 Sep 16.
Published in final edited form as: Cell Rep. 2019 Aug 6;28(6):1564–1573.e3. doi: 10.1016/j.celrep.2019.07.018

Figure 6. Interplay of Nej1 and Sgs1 in End-Resection Step of HR Repair Pathway.

Figure 6.

(A and B) Resection of DNA 0.15 kb away from the HO DSB, as measured by % ssDNA, at 0, 30, 60, 90, and 120 min post DSB induction in cycling cells. Strains used include WT (JC-727), nej1Δ (JC-1342), sgs1Δ (JC-3757), rad50sc+h (JC-4424), nej1Δ rad50sc+h (JC-4476), sgs1Δ rad50sc+h (JC-4478), nej1Δ sgs1Δ rad50sc+h (JC-4479) (A), and rad50sc+h N873I (JC-4561), nej1Δ rad50sc+h N873I (JC-4597), sgs1Δ rad50sc+h N873I (JC-4607), and nej1Δ sgs1Δ rad50sc+h N873I (JC-4605) (B). Analysis was performed in triplicate from at least three biological replicate experiments. Statistical analysis is described in STAR Methods.