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. 2019 Jul 26;294(37):13811–13821. doi: 10.1074/jbc.RA119.008353

Figure 2.

Figure 2.

ER stress results in decreased stability of β-catenin in RKO cells. A, RKO cells were treated with TM (10 μg/ml) (upper panel) or TG (1 μm) (lower panel) in the presence of control cell medium (LCM) or Wnt3a-producing cell medium (WCM) for the indicated time intervals. Immunoblot analysis of β-catenin and phosphorylation of elF2α are shown. Quantification of the expression level of β-catenin relative to α-tubulin is shown. B, RKO cells were preincubated with 5 μm MG132 or 50 μm CQ for 2 h before treatment with DMSO or 1 μm TG for another 12 h. Immunoblot analysis of β-catenin protein levels and quantification are shown in the bar graph after normalization to the value of nontreated LCM control cells. C, RKO cells were preincubated with 10 or 25 μm LiCl for 2 h and then treated by 1 μm TG for another 8 h. Immunoblot analysis of β-catenin protein levels and quantification are shown in the bar graph after normalization to the value of nontreated LCM control cells. All results represent three independent experiments.