Figure 5.

MDAMB231-MV–mediated transfer of miR221 promotes AKT/NF-κB-dependent migration and invasion of nonmetastatic MCF7 cells. A–D, to determine the role of miR221 transported from MDAMB231 to MCF7 cells via MV in inducing MCF7 cell migration, MDAMB231 cells were transfected with anti-miR221 beside Scrambled control miR followed by the addition of PAR2AP or FVIIa. MV were isolated and fused with MCF7. Alternatively, MCF7 cells were pre-transfected with anti-miR221 alongside Scrambled miR followed by the addition MDAMB231-derived MV. The migratory property of these MV-fused MCF7 cells was analyzed thereafter, which indicates that MDAMB231-MV–mediated induction of MCF7 migration was suppressed upon introduction of anti-miR221; scale bar 100 μm. E–H, to understand the contribution of MDAMB231-MV in promoting MCF7 invasion, the donor MDAMB231 cells were transfected with anti-miR221 followed by the addition of PAR2AP or FVIIa. MV were collected and incorporated into MCF7. Alternatively, MCF7 were pre-transfected with anti-miR221 followed by the fusion of MDAMB231-derived MV. MV-fused MCF7 cell invasion was measured by Transwell invasion assay; scale bar 100 μm, as mentioned briefly under “Experimental procedures.” The induction of MCF7 invasion by MDAMB231-MV was repressed upon administration of anti-miR221.