Figure 8.

Ega3 requires conserved acidic residues for GAG hydrolysis and anti-biofilm activity. A, cartoon representation of Ega3 showing the residues mutated in this study. B, point mutations of the conserved amino acids in the putative substrate-binding cleft were assayed for activity against A. fumigatus biofilms. No disruption indicates no activity against biofilms at the highest concentrations tested (100 μm). Text and bar colors correspond to the residue coloring as depicted in A. Bars indicate the mean of 2–8 assays performed in triplicate with the standard deviation of the logEC₅₀. Statistical significance as compared with WT was determined using one-way analysis of variance with Dunn's multiple comparison. *, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001.