Table 2.
Strains and primers for Ega3
fwd is forward; rev is reverse.
| Sequence or description | Source or Ref. | |
|---|---|---|
| Primers | ||
| 68NdeI | GGGCATATGGGTAATTATACCACCGCAAAATGGCAG | This study |
| 72NdeI | GGGCATATGACCGCAAAATGGCAGCCTGCAGTTGGC | This study |
| 75NdeI | GGGCATATGTGGCAGATTGAACTGCTGTATGCACTG | This study |
| 310HindIII | CCAAGCTTTTACAGGTTCATGTTTTTGATGACGGTGCT | This study |
| 318HindIII | CCAAGCTTCTCGAGTTAGCAATATTCCACCCA | This study |
| His fwd | GGGAGTCATCGTATGGGCAGCAGCCATCATCATCATC | This study |
| Ega3 rev | GGGGGTACCGCAATATTCCACCCA | This study |
| D189A fwd | GGTGTTGATCCGGCAAATGTGGATG | This study |
| D189A rev | CATCCACATTTGCCGGATCAACACC | This study |
| D189N fwd | GTGTTGATCCGAATAATGTGGATGG | This study |
| D189N rev | CCATCCACATTATTCGGATCAACAC | This study |
| E247Q fwd | GGTCAGTTAATCAACAGTGTGCCCAG | This study |
| E247Q rev | CTGGGCACACTGTTGATTAACTGACC | This study |
| D192A fwd | GATAATGTGGCAGGCTATGATAATG | This study |
| D192A rev | CATTATCATAGCCTGCCACATTATC | This study |
| D195A fwd | GTGGATGGCTATGCAAATGATAATG | This study |
| D195A rev | CATTATCATTTGCATAGCCATCCAC | This study |
| D232A fwd | GAAAAATGCCGGTGCAATTATTCCG | This study |
| D232A rev | CGGAATAATTGCACCGGCATTTTTC | This study |
| E133D fwd | GCAGGCAGCTATGATAATTGGCGTC | This study |
| E133D rev | GACGCCAATTATCATAGCTGCCTGC | This study |
| E157S fwd | GATGATTGGCCTGGTAGCAAATGGC | This study |
| E157S rev | GCCATTTGCTACCAGGCCAATCATC | This study |
| E254Q fwd | CAGTATAATCAGTGTGATACCTATG | This study |
| E254Q rev | CATAGGTATCACACTGATTATACTG | This study |
| W154A fwd | CACGATCTGGATGATGCACCTGGTG | This study |
| W154A rev | CATTTTTCACCAGGTGCATCATCCAG | This study |
| W154F fwd | CACGATCTGGATGATTTTCCTGGTG | This study |
| W154F rev | CATTTTTCACCAGGAAAATCATCCAG | This study |
| Y126F fwd | GTAAAGTTATTTGTTTTCTTAGCGC | This study |
| Y126F rev | GCGCTAAGAAAACAAATAACTTTAC | This study |
| Y273F fwd | GTTTCATATCGAATTTCCGAAAGGC | This study |
| Y273F rev | GCCTTTCGGAAATTCGATATGAAAC | This study |
| Strains | ||
| Af293 | Wildtype pathogenic strain of A. fumigatus | 7 |
| PA14 | Wildtype pathogenic strain of P. aeruginosa | 90 |
| Pichia pink strain 4 | P. pastoris laboratory expression strain: ade2, prb1, pep4 | Novagen |
| TOP10 | E. coli cloning strain | Invitrogen |
| BL21 CodonPlus | E. coli laboratory expression strain: F− ompT hsdS (rB− mB−) dcm+ Tetr galλ (DE3) endA [argU proL Camr] | Stratagene |
| Origami2 (DE3) | E. coli laboratory expression strain: Tetr Strrλ (DE3),trbX gor | Stratagene |