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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2019 Jun 6;378:114614. doi: 10.1016/j.taap.2019.114614

Fig. 3.

Fig. 3.

Over-expression of miR-186 in clones of HaCaT cells transfected with miR-186 expression plasmid or empty vector. Expression of miR-186 was determined by RT-qPCR of RNA isolated from 3 clones stably transfected with pEP-hsa-miR-186 Expression Vector (186) and 3 clones transfected with empty vector control plasmid (Ctrl) and incubated for 4 or 8 weeks with 0 or 100 nM sodium arsenite as indicated. The means and 25/75% confidence intervals are indicated by the line and box. Individual values are shown as dots. One pEP-hsa-miR-186 transfected clone expressed miR-186 at much higher level than the other two and lies above the confidence interval. The empty vector transfected clones all expressed miR-186 at about the same level (often overlapping) and lower than any of the pEP-hsa-miR-186 transfected clones. All values are normalized to the mean of the control cells without arsenite at 8 weeks.