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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Ocul Surf. 2019 Mar 16;17(4):793–808. doi: 10.1016/j.jtos.2019.03.002

Figure 13. Major species of OAHFA detected in mouse female and male tarsal plate extracts.

Figure 13.

All experiments were conducted in negative ion mode. Analytes were detected as (M − H) ions.

Panels A1 and A2. The extracted ion chromatogram and the mass spectrum of OAHFA with m/z 811.7549 (molecular formula C54H100O4). Note the presence of coeluting homologous ions 785.7396 and 837.7667.

Panels B1 and B2. The extracted ion chromatogram and negative ion mode mass spectrum of OAHFA with m/z 783.7238 (molecular formula C52H96O4). Note the presence of coeluting homologous ions 757.7052 and 809.7388.

Panels C1 and C2. The extracted ion chromatogram and negative ion mode mass spectrum of OAHFA with m/z 755.6944 (molecular formula C50H92O4). Note the presence of a coeluting homologous ion 781.7200.

Panel D. Profiles of OAHFA in female and male mouse tarsal plate samples (n=10, each group).

Panel E. The fragmentation pattern of the OAHFA C52H96O4. The major isoforms is shown.