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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Ocul Surf. 2019 Mar 16;17(4):793–808. doi: 10.1016/j.jtos.2019.03.002

Figure 4. Gas chromatographic-mass spectrometric analysis of wax esters extracted from mouse tarsi.

Figure 4.

Panel A. Extracted ion chromatograms of saturated wax esters. Each trace corresponds to a particular ion.

Panel B. Extracted ion chromatograms of FA residues formed due to the spontaneous in-source fragmentation of WE. Each trace corresponds to a particular ion.

Panel C. Relative abundances of FA in the WE fraction of mouse tarsal plate extracts. Note that saturated FA residues produced M+. ions, while unsaturated FA were visible as (M + H)+ adducts.

Panel D. Relative abundances of saturated and unsaturated WE in the mouse tarsal plate extracts and expressed meibum. The analytes were detected as M+. molecular ions (loss of electron). The results are shown as fractions (in %) of the total pool of analytes shown in the Figures.