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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Ocul Surf. 2019 Mar 16;17(4):793–808. doi: 10.1016/j.jtos.2019.03.002

Figure 7. Analysis of free cholesterol and cholesteryl esters using C18-UPLC/APCI-HRMS in positive ion mode.

Figure 7.

Panel A. Extracted ion chromatogram of ion m/z 369.35 produced by a mixture of authentic free Chl (peak 1), cholesteryl palmitoleate (peak 2), cholesteryl oleate (peak 3), cholesteryl erucate (peak 4), and cholesteryl nervonate (peak 5). Average peak area for all four CE was 1509582 arbitrary units; SD = 7.9%

Panel B. Extracted ion chromatogram of ion m/z 369.35 produced by a mixture of authentic free Chl (peak 1), and saturated straight chain CE: C7:0-Chl (peak 6), C9:0-Chl (peak 7), C11:0-Chl (peak 8), C12:0-Chl (peak 9), C14:0-Chl (peak 10), C15:0-Chl (peak 11), C16:0-Chl (peak 12), C18:0-Chl (peak 13), C20:0-Chl (peak 14), C22:0-Chl (peak 15), C23:0-Chl (peak 16), C24:0-Chl (peak 17). Average peak area for all twelve CE was 1554166 arbitrary units; SD = 6.0%.

Panel C. Extracted ion chromatogram of ion m/z 369.35 produced by a sample of expressed mouse meibum. Peak 1 – free Chl. Other peaks discussed later in the manuscript.

Panel D. Extracted ion chromatogram of ion m/z 369.35 produced by a sample of extracted mouse tarsal plate lipids.