Skip to main content
. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Ocul Surf. 2019 Mar 16;17(4):793–808. doi: 10.1016/j.jtos.2019.03.002

Figure 8. C18-UPLC/APCI-HRMS analysis of cholesteryl esters detected in tarsal plate extracts of a male mouse in positive and negative ion modes. The analysis is based on spontaneous in-source fragmentation of CE which produces an ion m/z 369.3521 (Chl − H2O + H)+ in PIM, and corresponding FA fragments (FA – H)− in NIM.

Figure 8.

Left panels. A fragment of an extracted ion chromatograms of ions m/z 369.35 (PIM) and five FA fragments m/z 393.37, 367.36, 381.37, 395.39, and 421.41 (NIM).

Right panels. High resolution mass spectra of fragments of cholesterol (m/z 369.35; PIM) and three FA fragments of CE m/z 393.37, 367.36, 381.37, 395.39, and 421.41 (NIM).