Fig. 4.

Evaluation of the potential role of APAF1 depletion on malignant progression. a Upper panel: Effects of APAF1 knockdown on apoptotic signaling was monitored via Annexin V assay using FACS, after treatment with 1 μM STS for 24 h. The numbers indicate the percentage of apoptotic cells in each quadrant. Lower panel: The values indicate the levels of apoptotic cells from the upper panel. b Increased cell viability of the indicated cells after STS treatment was evaluated by WST-1 assay (n = 3; bar represents the SE; *P < 0.05, **P < 0.05, Student’s t test. P values represent the indicated comparisons). c Migration assay was performed using Transwell chamber