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. 2019 Sep 16;9:13299. doi: 10.1038/s41598-019-49868-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Semiquantitative immunoblotting of MBP and CPT1A. Protein expression was investigated in the HB of WT EAE (n = 6) and Cpt1a P479L EAE mice (n = 6). Immunoblotting with MBP revealed four bands of 14.0, 17.0, 18.5 and 21.5 kDa (a). All detected MBP isoforms from Cpt1a P479L EAE mice were expressed at significantly higher levels than those from WT EAE mice (two-way ANOVA with Sidak’s multiple comparisons post hoc test) (b). Analysis of CPT1A protein expression revealed no significant differences between WT EAE and Cpt1a P479L EAE mice (c,d). The data were normalized to the density of the β-actin control. Data are presented as the mean ± SEM. Asterisks indicate the level of significance (**p < 0.01, ***p < 0.001, ****p < 0.0001). More information about full-length blots can be found in Supplementary Figs S1 and S2.