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. 2019 Sep 16;9:13390. doi: 10.1038/s41598-019-48822-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCA of global gene expression in PBMCs stimulated with PMA, LPS, and ionomycin under equi-nicotine conditions. Human PBMCs were pre-treated with low, medium or high dose WS-CM (0.1, 1.0, and 3.0 µg/mL equi-nicotine units, respectively) or STE (100 µg/mL equi-nicotine units) for three hours followed by stimulation with the agonists (PMA [50 ng/mL], ionomycin [500 ng/mL], and LPS [1 μg/mL]) for five hours. PCA was carried out using R. Gene expression level of all probe sets was dimensionally-reduced and the first top three principal components were presented. While the samples pretreated with high- and medium- WS-CM are distinct and far separated from the control, those pretreated with low WS-CM and STE are more similar and clustered closer to the control samples.