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. 2019 Sep 16;9:13390. doi: 10.1038/s41598-019-48822-w

Figure 5.

Figure 5

Cytokine expression. PBMCs were pretreated with different concentrations of WS-CM or 100 µg/mL equi-nicotine units of STE for 3 hours and stimulated with the agonists for 24 hours. Levels of cytokines in the culture supernatants were determined using a cytometric bead array and flow cytometer. Each point represents the mean ± SD error bars of four donors from a representative experiment. Since the 3 cytokine levels with high dose treatment and the TNF levels in medium dose WS-CM were below the limits of detection, lowest detectable values were used to compute statistical significance. The statistical significance was indicated by: *P < 0.05 and **P < 0.005.