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. 2019 Sep 16;9:13393. doi: 10.1038/s41598-019-49706-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The validation analysis of miR146b-5p expression and the identification of responsive immune cells producing hsa-miR146b-5p. The quantification of miR146b-5p in the serum was carried out to validate the comprehensive analysis using real-time PCR. The Cel-miR-39-3p was spiked in the serum for the control miRNA. The relative expressions of miR146b-5p are shown in the Y-axis. One healthy subject indicated one relative expression. We normalized the other subjects using the relative expression (A). Then, we analyzed the expression of hsa-miR146b-5p in various kinds of isolated immune cells (PBMCs, CD3⁺ T cells, CD14⁺ monocytes, CD19⁺ B cells and CD56⁺ NK cells). The quantification of miR146b-5p in the various kinds of cells was carried out using real-time PCR. One PBMC sample in a healthy subject indicated one relative expression. Then, we normalized the other samples using the relative expression. The relative expressions of miR146b-5p are shown in the Y-axis (B). A comparison of hsa-miR146b-5p expression in monocytes between IL28B T/T (n = 26) and non-IL-28B T/T (n = 21) patients was carried out (C). A comparison of hsa-miR146b-5p expression between the SVR patients (n = 10) and non-SVR patients (n = 10) after receiving PEG-IFN/RBV treatment was carried out (D). A comparison of hsa-miR146b-5p expression between the SVR patients (n = 10) and non-SVR patients (n = 7) after receiving DCV/ASV treatment was carried out (E). Error-bars indicate standard deviation. The expression levels of hsa-miR146b-5p in CD14⁺ monocytes were compared between before and after achieving SVR. (F) The relative expressions of miR146b-5p are shown in the Y-axis.