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. 2019 Sep 16;9:13291. doi: 10.1038/s41598-019-49479-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression stability and optimal number for normalization of candidate reference genes analyzed by geNorm. (A–D) The stability M values of candidate genes in tissues, developmental stages, sexes and all sample, respectively. (E–H) The pairwise variations V_n/V_{n+ 1} of candidate genes in four treatments mentioned above.