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. 2019 Sep 16;9:13291. doi: 10.1038/s41598-019-49479-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of the gene stability measures. Expression levels of a target gene, OBP3, in six tissues (A) and all samples (including two tissues, two developmental stages, and two sexes) (B) were tested by different normalization reference genes. Bars represent the means and standard deviations of three biological replicates. Single asterisk indicates significant differences among different treatment within OBP3 expression by normalized by reference gene or combination (P < 0.05, Duncan’s test), and double asterisks show extremely significant differences in the expression of OBP3 (P < 0.01, Duncan’s test).