Table 1.

Primer amplification characteristics of candidate genes for qRT-PCR in H. theivora.

Gene (Abbreviation)	Accession Number	Primer sequence	Product length (bp)	Tm (°C)	R 2	E (%)
Actin	MN027587	5′ TACCGCAAGATTCCATACCCA 3'	109	60	0.999	90.58
		5′ AAGTCCTACGAGCTTCCCGAC 3'
β-Tubulin1	MN027588	5′ GATGAAGGAAGTTGACGAGCA 3'	237	57	0.999	90.10
		5′ TCACCAGTATACCAATGGAGG 3'
β-Tubulin2	MN027589	5′ TCAGGAAAAGGAGGCGGCAAA 3'	365	61	0.999	90.99
		5′ GGCGAGGGAACGACAGAGTAG 3'
RPL13A	MN027592	5′ ACACAAAATCGAAAGGGGAAA 3'	153	59	1.000	93.76
		5′ CGACCAAGATGACAGTAGGCA 3'
EF1α	MN027594	5′ TCGCTTTCGTTCCAATCTCTG 3'	107	60	0.999	91.66
		5′ GGCTTTGCCTTCTTTCCTTTC 3'
RPS3A	MN027598	5′ GCAGCATGGTGAAAAAGTGGC 3'	151	60	0.999	91.21
		5′ ACCTGGTTGTGTTGAGCGTAA 3'
GAPDH	MN027590	5′ CAATGACCCCTTTTTGGGCG 3'	163	60	0.999	96.69
		5′ CCATGGGATGGCTTTGGGAT 3'
18SRNA	MN027591	5′ GCGATGTTGAACGTTGCTGT 3'	110	59	0.999	96.32
		5′ ACGCTTATGGCTCCGTCAAA 3'
G6PDH	MN027593	5′ CGTCTGCATATAGCGTCCGT 3'	237	60	0.999	95.01
		5′ GAGAACCACGTCGTCCACAT 3'
EIF4A	MN027595	5′ TGGTTCGAAACATGCCGAGA 3'	184	60	0.999	93.64
		5′ GCGTTGCTGAATAGCGGAAG 3'
TBP	MN027596	5′ CATCCCCAGCATCGGTACTC 3'	296	60	0.999	93.79
		5′ AAGGCGTCATCGGTGTCATT 3'
UBQ	MN027597	5′ GATTATCCGCTGAGCCCTCC 3'	226	60	1.000	96.87
		5′ CATTAGCGCCACTTTCGTCG 3'