Table 2.

Expression stability ranking of the twelve candidate reference genes.

Experimental conditions	Methods	Stability ranking
		1	2	3	4	5	6	7	8	9	10	11	12
Tissues	geNorm	RPL13A/RPS3A	Actin	UBQ	EF1α	EIF4A	GAPDH	TBP	18SRNA	β-Tubulin2	G6PDH	β-Tubulin1
	NormFinder	RPS3A	Actin	UBQ	EF1α	RPL13A	GAPDH	EIF4A	TBP	β-Tubulin2	18SRNA	G6PDH	β-Tubulin1
	Delta Ct	RPS3A	Actin	UBQ	EF1α	RPL13A	EIF4A	GAPDH	TBP	β-Tubulin2	18SRNA	G6PDH	β-Tubulin1
	Bestkeeper	G6PDH	Actin	RPL13A	UBQ	RPS3A	EIF4A	18SRNA	GAPDH	EF1α	TBP	β-Tubulin2	β-Tubulin1
	RefFinder	RPS3A	Actin	RPL13A	UBQ	EF1α	G6PDH	EIF4A	GAPDH	TBP	18SRNA	β-Tubulin2	β-Tubulin1
Developmental stages	geNorm	EF1α/EIF4A	GAPDH	RPS3A	RPL13A	G6PDH	β-Tubulin1	UBQ	18SRNA	TBP	Actin	β-Tubulin2
	NormFinder	RPL13A	GAPDH	β-Tubulin1	EIF4A	RPS3A	EF1α	UBQ	18SRNA	G6PDH	TBP	Actin	β-Tubulin2
	Delta Ct	RPL13A	GAPDH	EIF4A	EF1α	RPS3A	β-Tubulin1	UBQ	18SRNA	G6PDH	TBP	Actin	β-Tubulin2
	Bestkeeper	UBQ	18SRNA	β-Tubulin1	RPL13A	Actin	β-Tubulin2	GAPDH	TBP	G6PDH	RPS3A	EIF4A	EF1α
	RefFinder	RPL13A	GAPDH	EIF4A	EF1α	UBQ	β-Tubulin1	18SRNA	RPS3A	G6PDH	Actin	TBP	β-Tubulin2
Sexes	geNorm	RPL13A/RPS3A	EIF4A	EF1α	UBQ	β-Tubulin1	18SRNA	GAPDH	Actin	G6PDH	TBP	β-Tubulin2
	NormFinder	EF1α	Actin	RPL13A	EIF4A	RPS3A	GAPDH	UBQ	18SRNA	G6PDH	β-Tubulin1	TBP	β-Tubulin2
	Delta Ct	EF1α	RPL13A	EIF4A	RPS3A	UBQ	GAPDH	Actin	18SRNA	β-Tubulin1	G6PDH	TBP	β-Tubulin2
	Bestkeeper	β-Tubulin1	UBQ	EIF4A	RPS3A	RPL13A	GAPDH	EF1α	G6PDH	18SRNA	Actin	TBP	β-Tubulin2
	RefFinder	EF1α	RPL13A	RPS3A	EIF4A	UBQ	β-Tubulin1	Actin	GAPDH	18SRNA	G6PDH	TBP	β-Tubulin2
All samples	geNorm	RPL13A/RPS3A	EIF4A	EF1α	GAPDH	UBQ	18SRNA	TBP	G6PDH	β-Tubulin1	β-Tubulin2	Actin
	NormFinder	RPL13A	EIF4A	RPS3A	EF1α	18SRNA	UBQ	GAPDH	TBP	β-Tubulin1	G6PDH	β-Tubulin2	Actin
	Delta Ct	RPL13A	EIF4A	RPS3A	EF1α	UBQ	GAPDH	18SRNA	TBP	β-Tubulin1	G6PDH	β-Tubulin2	Actin
	Bestkeeper	RPL13A	β-Tubulin1	RPS3A	EIF4A	18SRNA	Actin	TBP	UBQ	EF1α	GAPDH	β-Tubulin2	G6PDH
	RefFinder	RPL13A	RPS3A	EIF4A	EF1α	18SRNA	UBQ	β-Tubulin1	GAPDH	TBP	Actin	G6PDH	β-Tubulin2