Fig. 3. The novel isoforms show a higher kinetic stability in their dimeric state compared to TAp63α.

a–c SEC analysis of TAp63α, TA*p63α and GTAp63α FWL expressed in rabbit reticulocyte lysate. Lysates were applied on a Superose 6 PC 3.2/30 column. Fractions were collected, analyzed and quantified via western blot. The sum of the intensities of all fractions corresponds to 100%. Experiments were performed five times, error bars indicate SD. d Urea BN-PAGE followed by western blotting for myc-tagged TAp63α, TA*p63α and GTAp63α. Two nanogram expression vector carrying the p63 gene were transiently transfected in H1299 cells (10 cm dish). Cells were harvested 24 h after transfection. Lysates were incubated with different urea concentrations on ice and applied on the gel. Migration of the different oligomeric states is indicated by D (dimer) and M (monomer). Monomers appear on the gel due to the intrinsic kinetically instability of the p63 tetramerization domain³⁹