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. 2019 Sep 16;9:13308. doi: 10.1038/s41598-019-49809-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Basal levels of p-Akt and p-MEK in TNBC and drug resistance. (A) Western blot analysis and (B) quantification of p-Akt and p-MEK levels in the Akt-i-resistant, MEK-i-resistant and double-resistant cell lines. P-Akt and p-MEK levels were normalised to tubulin. Data are expressed as means ± SD of two independent experiments (two-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Full-length uncropped blots are shown in Supplementary Fig. S4.