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. 2019 Sep 16;9:8012. doi: 10.1038/s41598-019-44304-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activity of compounds against MRSA strains. (A) Confirmation of MRSA strains. MIC values for MSSA (ATCC 25923) and six MRSA strains were analyzed by the concentration diffusion assay using an E-test on MHA plate. Arrow indicates the MIC of oxacillin for individual strains. The data shown are representatives of n = 3. (B) PCR confirmation of the mecA gene. PCR amplification using mecA-specific primer sets was performed for MSSA (ATCC 25923, Lane 1) and 6 MRSA strains (Lanes 2–7). M indicates DNA size marker (GeneRuler 100 bp Plus DNA ladder; Thermo Scientific). Asterisk (*) indicates non-specific PCR product. (C) Growth analysis of MRSA strains against compounds and streptomycin. Different concentrations of compounds were added to ATCC 25923 cells and cultured for 24 h. One representative experimental data from n = 5 was shown. (D) Determination of MIC. The values (μg/mL) were averaged from n = 5. All photographed images shown in (A) to (C) were processed in Adobe Illustrator CS6 (v16.0.0, Adobe Systems Inc., USA).