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. 2019 Sep 16;9:13415. doi: 10.1038/s41598-019-50103-5

Figure 11.

Figure 11

Effects of BBR on firefly luciferase activities of recombinant plasmids containing Gal-3 3′UTR. Recombinant dual-luciferase expressing plasmids containing wild type (Wt) or a point mutation (Mut) of mouse Gal-3 3′UTR were transfected into mouse primary preadipocytes. After 24 h of transfection, cells were treated with DMSO or different concentrations of BBR for 24 h. (A) Alternatively, cells were induced for differentiation with MDI for 24 h after transfection, and meanwhile DMSO or BBR were added and incubated. (B) After treatment, the activities of firefly luciferase were determined, normalized to Renilla luciferase activities, and presented as fold of control cells. Values are mean ± SD of 4 separate experiments. (A) *p < 0.05, **p < 0.01 vs. that of DMSO; #p < 0.05, ##p < 0.01 vs. that of pmirGLO-Gal-3–3′UTR-Wt transfected cells. (B) **p < 0.01 vs. that of preadipocytes; #p < 0.05, ##p < 0.01 vs. that of cells treated with MDI + DMSO; $p < 0.05, $$p < 0.01 vs. that of pmirGLO-Gal-3-3′UTR-Wt transfected cells.