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. 2019 Sep 16;9:13415. doi: 10.1038/s41598-019-50103-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of BBR on eWAT Gal-3 expression in mice fed a HFD. Male C57BL/6 J mice were grouped and treated as described in “Methods”. At the end of experiment, mice were sacrificed, and eWATs were subjected to paraffin sectioning. After dewaxing, Gal-3 protein expression levels were analyzed by IHC staining(×200, scale bar = 400 μm) (A, upper panel) and integrated optical density (IOD) values (A, lower panel) were determined. Meanwhile, Gal-3 mRNA expression levels in eWATs were analyzed by real-time RT-PCR, normalized to ACTB, and presented as fold of ND group (B). Values are mean ± SD of 10 mice in each group. **p < 0.01, ***p < 0.001 vs. that of ND group; ^##p < 0.01, ^###p < 0.001 vs. that of HFD group.