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. 2019 Sep 16;9:13415. doi: 10.1038/s41598-019-50103-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of BBR on Gal-3 expression during differentiation of mouse primary preadipocytes. Mouse primary preadipocytes were left untreated or induced for differentiation. During induction, cells were left untreated or treated with BBR at 20 μmol/L for different time intervals, and cells were harvested for western blot (A) or real time RT-PCR (B) analysis of Gal-3 protein or mRNA levels. Alternatively, cells were left untreated or treated with different concentrations of BBR during the 10 days of differentiation period, and Gal-3 protein (C) and mRNA (D) levels were determined. The expression levels of Gal-3 were normalized to ACTB and presented as fold of preadipocytes. Values are mean ± SD of 3–4 separate experiments. *p < 0.05, **p < 0.01 vs. that of preadipocytes; ^#p < 0.05, ^##p < 0.01 vs^. that of differentiated adipocytes left untreated.