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. 2019 Sep 16;9:13415. doi: 10.1038/s41598-019-50103-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Gal-3 silencing on the differentiation of mouse primary preadipocytes. Mouse primary preadipocytes were transfected with Gal-3 or control siRNA and induced for differentiation for 6 days. Cells were harvested for western blot analysis of Gal-3 protein levels (A), intracellular TG (B) and the mRNA levels of adipogenic genes (C) were also determined. The protein or mRNA levels of target genes were normalized to ACTB and presented as fold of preadipocytes. Values are mean ± SD of 3–4 separate experiments. **p < 0.01, ***p < 0.001 vs. that of preadipocytes; ^###p < 0.001 vs. that of differentiated adipocytes left untransfected or transfected with control siRNA.