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. 2019 Sep 16;9:13415. doi: 10.1038/s41598-019-50103-5

Figure 8.

Figure 8

Effects of Gal-3 overexpression on the activities of BBR in mouse primary preadipocytes. Mouse primary preadipocytes were left untreated or transfected with Gal-3 or Control CRISPR Activation Plasmids as indicated. After 24 h of transfection, cells were left untreated or treated with 20 μmol/L of BBR for 48 h. After treatment, Gal-3 protein (A) and mRNA (B) levels were determined, which were normalized to ACTB and presented as fold of control cells. In parallel experiments, BrdU incorporation (C) and cell numbers (D) were determined. Values are mean ± SD of 3–4 separate experiments. *p < 0.05, **p < 0.01 vs. that of untransfected or control plasmid transfected cells not treated with BBR; $$p < 0.01, $$$p < 0.001 vs. that of untransfected or control plasmid transfected cells treated with BBR.