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. 2019 Sep 17;10(10):683. doi: 10.1038/s41419-019-1930-5

Fig. 5. Necroptosis marker p-MLKL can be detected in OA cartilage and after TNF/CHX stimulation.

Fig. 5

Exemplary images of impacted and differently stimulated cartilage explants after immunohistochemical staining of a cleaved CASP8, b RIPK3, and c p-MLKL. In addition, percentage of cleaved CASP8− (d) and p-MLKL− (e) positive cells was quantified (n = 3). Images were acquired using a 20× objective; the bars represent 200 µm