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. 2019 Sep 16;9:13295. doi: 10.1038/s41598-019-49840-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of NE activity in oral cancer tissues. (A) Lysates prepared from normal mouse tongues or HSC-3 oral cancer tissues were labeled with 1 µM PK105b and analyzed by in-gel fluorescence (n = 3–5). *Indicates high-molecular weight species of unknown identity. (B) Total NE expression in the samples in (A) was measured by immunoblotting with an NE-specific antibody. (C,D) Densitometry analysis of active NE in (A) and total mature NE in (B), respectively. (E) Ratio of active to total mature NE in the samples. Error bars are shown as mean ± SEM. (F) NE labelling in cancer tissue was confirmed by immunoprecipitation with an NE-specific antibody. (G) Competition of PK105b labeling in oral cancer tissue lysate with PK101.