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. 2019 Sep 16;9:13295. doi: 10.1038/s41598-019-49840-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Luminal protease activity in acute experimental colitis. (A,B) Labeling of luminal fluids or fecal pellets from control or TNBS-treated mice with PK105b. (C) Immunoprecipitation of PK105b-labeled fecal samples with NE, PE, or trypsin 3-specific antibodies. Gain settings for the pulldowns were enhanced in order to observe faint bands (right panel).