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. 2019 Sep 10;10:2065. doi: 10.3389/fimmu.2019.02065

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Copyright © 2019 Daza-Cajigal, Albuquerque, Pearson, Hinley, Mason, Stahlschmidt, Thrasher, Mishra, Southgate and Burns.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of MHC I/II, ICAM-1 and PD-L1 expression in hTERT urothelial cells by Flow Cytometry. Flow cytometry analysis of surface receptor expression in KD and Sc hTERT immortalized urothelial cell lines before and after IFNγ stimulation. Mean fluorescence intensity and percentage of positive cells are shown for (A,B) MHC II (C,D) MHC I (E,F) ICAM-1, and (G,H) PD-L1 expression. Data is from four independent experiments for (A–F) and from three independent experiments (G,H). One-way ANOVA with Tukey's Multiple Comparison post-test. Graphs shows mean values ± SE and regulation of the expression compared to untreated. ^*P < 0.05. Error bars represent the SE.