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. 2019 Sep 10;10:2065. doi: 10.3389/fimmu.2019.02065

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Copyright © 2019 Daza-Cajigal, Albuquerque, Pearson, Hinley, Mason, Stahlschmidt, Thrasher, Mishra, Southgate and Burns.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of IFNγ-regulatory genes and intermediary transcription factors in NHU cells by RT-qPCR. (A–D) RT-qPCR analysis of gene expression in five non-immortalized NHU cell cultures obtained from five different donors, with or without IFNγ (200 U/ml) and/or TZ (1 μM) + PD153035 (1 μM) stimulation for 48 h compared with the vehicle-only (0.1% DMSO) control. Data are from five independent experiments each using a different NHU cell donor. One-way ANOVA with Tukey's Multiple Comparisons Test. ^*P < 0.05. Error bars represent the SE.