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. 2019 Sep 10;9:316. doi: 10.3389/fcimb.2019.00316

Figure 1.

Figure 1

Transcriptional analyses of rhoptry genes in sporozoites and schizonts. Total RNA was extracted from parasite-infected mosquito midguts at days 9, 13, and 17 post-feeding (MG SPZ D9, MG SPZ D13, and MG SPZ D17); and salivary glands at day 17 post-feeding (SG SPZ D17). Total RNA was also extracted from schizont-enriched infected erythrocytes (Schizont). The mean values of relative mRNA amounts of each molecule, normalized by ef1α mRNA expression, are plotted as bar graphs with standard deviations from three independent experiments. (A) A group of molecules categorized as rhoptry neck proteins in merozoites. All examined molecules are transcribed in both merozoites and sporozoites. (B) A group of molecules categorized as rhoptry bulb proteins in merozoites whose transcriptions are detected in both merozoites and sporozoites. (C) A group of molecules categorized as rhoptry bulb proteins in merozoites whose transcriptions occur dominantly in merozoites. (D) Transcription profile of a typical micronemal protein, SPECT2, required for sporozoite migration toward hepatocytes after inoculation in the skin. Its transcript level drastically increases after sporozoite invasion of salivary glands.