Figure 3.

Expression pattern of rhoptry proteins in merozoites and sporozoites. Schizonts and sporozoites collected from midguts or salivary glands at day 24–26 post-feeding were fixed with acetone on glass slides. In schizonts, anti-c-Myc antibodies were used to detect target c-Myc fused rhoptry protein (shown in green) in each transgenic parasite, which is compared with the localization pattern of RON2 (shown in red) and nuclei (blue) stained by anti-RON2 antibodies and DAPI, respectively. To detect ASP/RON1 and RAMA, RON2-c-Myc transgenic parasites were used as antigens and target proteins and RON2 was detected by specific antibodies (green) and anti-c-Myc antibodies (red). Differential interference contrast (DIC) images are shown in the left panels. In sporozoites (right two panels), the localization of target proteins (green) and nuclei (blue) are shown in the merged images. Bar indicates 5 μm.