Skip to main content
. 2019 Sep 10;10:2143. doi: 10.3389/fimmu.2019.02143

Figure 1.

Figure 1

Basophils show a variable phenotype during inflammatory conditions. (A) Contour plots comparing basophil phenotypes among splenocytes of B8xC57 mice infected by H. polygyrus for 15 days or not (n = 3–4), and their expression of FceRIa and eYFP or (B,C) among total ear skin cells during 12 days after treatment with MC903 (n = 10–11). (B) The expression of CD200R3 and eYFP by eYFP+ basophils is depicted overtime. (C) The expression of CD11b by and the proportion of Ly6C+ eYFP+ basophils is shown (D) Overlay of eYFP+ basophils and CD45+ leucocytes including, CD3+ CD4+ T cells (CD4+), CD11b+ Ly6G+ Neutrophils, SSChi SiglecF+ Eosinophils, CD64lo Ly6G- SiglecF- eYFP- CD11b+ Ly6C+ inflammatory monocytes (Ly6C+ monocytes). (E) Proportion of eYFP+ basophils expressing Ly6C after 24 h of stimulation of whole splenocytes from Basoph8 mice with 1 ng/mL of IL3 (n = 9). Data are representative of 2 (B–E) or 3 (A) independent experiments giving similar results. Statistics are unpaired t-test (A) or one-way ANOVA with a Dunnett's comparison to D0 (B,C). Ns, Non-significant; **p < 0.01; ****p < 0.0001.