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. 2019 Sep 10;10:2143. doi: 10.3389/fimmu.2019.02143

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Copyright © 2019 Pellefigues, Mehta, Prout, Naidoo, Yumnam, Chandler, Chappell, Filbey, Camberis and Le Gros.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Basophils are activated by conventional FACS staining. (A) Representative contours or dot plots showing eYFP+ basophils gating strategy and the expression of IL4 (AmCyan), IL13 (dsRed), or CD63 (antibody staining) by stimulated basophils. Whole splenocytes from B8x4C13R mice were stimulated for 24 h with (B) 2.4G2, (C) MAR1, or (D) Ba13 (n = 6). (E) Contours and dots plots depicting the sorting strategy and the purity of eYFP+ basophils from the spleen of B8xC57 mice infected with H. polygyrus. Data are from at least two independent experiments giving similar results. Statistical analyses are paired t-test. Ns, Non-significant; ^**p < 0.01; ^***p < 0.001; ^****p < 0.0001.