Figure 5.

Basophils from B8xiDTR mice can be specifically depleted ex vivo. (A) eYFP⁺ Basophils, Ly6G⁻ SiglecF⁺ SSC^hi eosinophils, B220⁺ MHCII⁺ B cells, CD3⁻NK1.1⁺ NK cells, CD11b⁺ Ly6G⁺ Neutrophils, CD3⁻ SiglecF⁻ Ly6G⁻ CD11b⁺ CX3CR1⁺ Ly6C⁻ or Ly6C⁺ monocytes, CD3⁺ CD4⁺ or CD8⁺ T cells, and CD3⁻ B220⁺ CD11c⁺ CD11b⁻ Ly6C⁺ pDCs numbers were quantified by flow cytometry after stimulation for 24 h of whole femur bone marrow of B8xiDTR or B8xC57 mice with increasing doses of DT (n = 6). (B) eYFP⁺ Basophils, SiglecF⁺ SSC^hi eosinophils, B220⁺ MHCII⁺ B cells, CD3⁻NK1.1⁺ NK cells, CD11b⁺ Ly6G⁺ Neutrophils, CD3⁻ SiglecF⁻ Ly6G⁻ CD11b⁺ Ly6C⁺ monocytes, CD3⁺ CD4⁺ or CD8⁺ T cells, and CD3⁻ B220⁻ CD11c⁺ MHCII^hi DCs numbers were quantified by flow cytometry after stimulation for 24 h of whole splenocytes of B8xiDTR or B8xC57 mice with increasing doses of DT (n = 6). (C) Peritoneal lavages of B8xiDTR mice were stimulated or with 10 μg/mL of DT for 24 h and the number of CD64+ Macrophages, CD19⁺ B cells and CD200R3⁺ cKit⁺ Mast cells were quantified by flow cytometry. Data are from two independent experiments giving similar results (n = 8). (A,B) Data has been normalized on the unstimulated condition and represents only the B8xiDTR genotype. Statistical analyses are (A,B) a two-way ANOVA with a Sidak post-test comparing matched stimulated with unstimulated conditions or (C) a paired t-test. Ns, Non significant; ^*p < 0.05; ^****p < 0.0001.